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H3159
Sigma
Anti-Histone Deacetylase 2 (HDAC2) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
| MDL number: | MFCD02096544 |
Description
| Immunogen | synthetic peptide (C-SGEKTDTKGTKSEQLSNP) corresponding to the C-terminus region of histone deacetylase 2 of human origin (amino acids residues 471-488 with N-ternimal added cysteine) conjugated to maleimide-activated KLH. This sequence is highly conserved in mouse and chicken (2 amino acids and 1 amino acid substitutions, respectively). |
| Physical form | Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | IgG fraction of antiserum |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt 55 kDa |
| species reactivity | mouse, human |
| application(s) | immunoblotting: 1:20,000 using nuclear extract from human HeLa cells |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested, human lymph node sections | |
| immunoprecipitation: 1:2,500 using whole lysate of NIH3T3 cells | |
| microarray: suitable | |
| Gene Information | human ... HDAC2(3066) |
| mouse ... Hdac2(15182) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
